Journal: Cell reports

Article Title: Neurokinin-1 Receptor Signaling Is Required for Efficient Ca 2+ Flux in T-Cell-Receptor-Activated T Cells

doi: 10.1016/j.celrep.2020.02.054

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Anti-mouse / rat / human Substance P (rabbit polyclonal), Cy3 , Bioss USA , Cat # bs-0064R-Cy3.

Techniques: Functional Assay, Recombinant, Staining, Avidin-Biotin Assay, Blocking Assay, Plasmid Preparation, Inhibition, Fluorescence, SYBR Green Assay, Activation Assay, Bicinchoninic Acid Protein Assay, In Situ, Enzyme-linked Immunosorbent Assay, Activity Assay, Labeling, Software

Journal: Journal of Cellular and Molecular Medicine

Article Title: Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

doi: 10.1111/jcmm.12548

Figure Lengend Snippet: TCs immunodiagnostics by double-labelled immunofluorescence. Dotted arrows indicated CD34-positive vascular endothelial cells. Negative c-kit staining was not shown here; scale bar = 20 μm. (A) CD34 (red) in moniliform cells overlying vimentin (green) cells with DAPI counterstaining (blue) in sham control (solid arrows), indicated the existence of perivascular TCs with special immunophenotype of CD34/vimentin double-positive. (B and C) CD34/vimentin double-positive cells with specific TCs morphology and well-defined nuclei was significantly less densely stained, reduced, sparse or completely absent (solid arrows) in AS-affected oviduct tissues. A statistically significant decrease in the mean number of TCs occurred ( P = 0.000). * P < 0.05 versus sham control; error bars = SD.

Article Snippet: Then FITC-goat antimouse IgG for vimentin (1:50; cat no. BA1101), CY3-goat anti-rabbit IgG for CD34 (1:50; cat no. BA1032), TRITC-goat anti-rabbit IgG for c-kit (1:50; cat no. BA1090) were added (all from Boster).

Techniques: Immunofluorescence, Staining, Control

Journal: Journal of Cellular and Molecular Medicine

Article Title: Ultrastructure damage of oviduct telocytes in rat model of acute salpingitis

doi: 10.1111/jcmm.12548

Figure Lengend Snippet: TCs and Tps damage in AS-affected oviduct tissues, accompanied by excessive amount of collagen fibres (Coll) and tissue fibrosis. (A) Intercellular connection between damaged TCs and activated mononuclear cells (MC). (a) Degenerated Tps established closed contact to activated MC which contained dense secretory granules, together with granulocyte infiltration, mainly eosinophils (Eo) and neutrophils (PMN). b higher magnification of the boxed area; (b) synapse (black arrows) between activated MC and degenerated Tps, which contained lots of swollen mitochondria (m) and vacuoles (white arrows), thus indicating degeneration, functional insufficiency of TCs and involvement of TCs in local immunoregulation. (B) Degeneration, discontinue or dissolution of TCs and Tps (black arrows), with cytoplasmic vacuolization (white arrows), accompanied by nearly normal scattered putative stem cells (SCs). Intercellular contacts between TCs and SCs was getting wider or disappeared (black asterisks). (C) Disrupted TC-SC niches which composed of a group of damaged Tps and putative SCs in myosalpinx, with heterocellular contacts getting wider or disappeared between Tps and SCs (black asterisks), Tps and activated Eosinophils (Eo) (white asterisk) with dense secretory granules respectively. Degeneration, discontinue or dissolution of TCs and Tps (black arrows), with swollen mitochondria (m), cytoplasmic vacuolization (white arrows) in Tps, swollen and dissolution of SMCs can be observed. (D) Severely damaged perivascular TCs and Tps, with swollen mitochondria (m), rough endoplasmic reticulum (rER) dilatation and cytoplasmic vacuolization (white arrows), together with damaged endothelial cell (E) and pericytes (P).

Article Snippet: Then FITC-goat antimouse IgG for vimentin (1:50; cat no. BA1101), CY3-goat anti-rabbit IgG for CD34 (1:50; cat no. BA1032), TRITC-goat anti-rabbit IgG for c-kit (1:50; cat no. BA1090) were added (all from Boster).

Techniques: Functional Assay, Dissolution

Journal: Medical research archives

Article Title: Identification of amacrine neurons with a glycinergic and GABAergic phenotype in the mouse retina

doi: 10.18103/mra.v10i1.2624

Figure Lengend Snippet: List of antibodies used in this study

Article Snippet: Anti-Mouse (CY3) , Donkey , Jackson Immunoresearch Laboratories, 715–165-150 , .

Techniques:

Journal: Medical research archives

Article Title: Identification of amacrine neurons with a glycinergic and GABAergic phenotype in the mouse retina

doi: 10.18103/mra.v10i1.2624

Figure Lengend Snippet: A) Vertical sections from the central retina of the pGlyT2-eGFP mouse were subjected to immunostaining and analyzed by confocal microscopy. Z-stack images were acquired and an optical section from the middle of the stack selected for presentation. Left panel: bright field showing the retinal layers; middle panel, eGFP expression; right panel, merge image. Scale bar, 20 μm. B) Vertical section stained with anti-Syntaxin 1 (HPC-1) antibodies and DAPI followed by CY-3 labeled secondary antibodies. C) Same as B but sections labeled with rabbit antibodies against GlyT1 or D) anti-glycine. Scale bar, 10 μm. Abbreviations: INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. E) Quantitation of EGFP-positive or EGFP/GlyT1 neurons. Cell counts were obtained from a minimum of 100 HPC-1 positive cells per retina, for a total of three retinas from three different mice. The plots represent the percent of HPC-1 positive cells +/− SE. Open arrowhead in C and D indicate eGFP labeled cells devoid of GlyT1or Glycine immunoreactivity, respectively.

Article Snippet: Anti-Mouse (CY3) , Donkey , Jackson Immunoresearch Laboratories, 715–165-150 , .

Techniques: Immunostaining, Confocal Microscopy, Expressing, Staining, Labeling, Quantitation Assay

Journal: Medical research archives

Article Title: Identification of amacrine neurons with a glycinergic and GABAergic phenotype in the mouse retina

doi: 10.18103/mra.v10i1.2624

Figure Lengend Snippet: A) Vertical sections from the central retina of the pGlyT2-eGFP mouse were immunolabeled with antibodies recognizing the GABA synthetizing enzyme GAD67 and rabbit GlyT1 antibodies or synaptic systems or followed by the corresponding secondary CY-3 and CY-5 labeled antibodies. Z-stack images were acquired through the corresponding channels and an optical section from the middle of the stack selected and used for this figure panels. B) Sections were stained as in A but using the rabbit GABA transporter anti-GAT1 and rat anti-GlyT1 antibodies or C) GAT3 and rat GlyT1. Scale bar 10 μm. Abbreviations: INL, inner nuclear layer; IPL, inner plexiform layer. Open arrowhead in A-C indicate eGFP labeled cells containing GlyT1 and GAD67, or GAT1 or GAT3. D) A minimum of 100 HPC-1 positive cells per retina from three different eyes were used to quantify the number of GAD67 or GlyT1 positive cells and those containing both markers. The plots represent the percent of GAD67 or/and GlyT1 positive cells +/− SE. E) Same as D but normalized by 100 EGFP positive ACs.

Article Snippet: Anti-Mouse (CY3) , Donkey , Jackson Immunoresearch Laboratories, 715–165-150 , .

Techniques: Immunolabeling, Labeling, Staining

Journal: Medical research archives

Article Title: Identification of amacrine neurons with a glycinergic and GABAergic phenotype in the mouse retina

doi: 10.18103/mra.v10i1.2624

Figure Lengend Snippet: Vertical section of the central mouse retina were labeled with antibodies against the protein gephyrin (upper panel) or the GlyR subunits using the mAb4a antibody that recognizes all the receptor subunits, followed by staining with CY-3 labeled secondary antibodies. Confocal Z-stack images were acquired and an optical section from the middle of the stack selected and used for presentation. Scale bar, 10 μm.

Article Snippet: Anti-Mouse (CY3) , Donkey , Jackson Immunoresearch Laboratories, 715–165-150 , .

Techniques: Labeling, Staining

Journal: Medical research archives

Article Title: Identification of amacrine neurons with a glycinergic and GABAergic phenotype in the mouse retina

doi: 10.18103/mra.v10i1.2624

Figure Lengend Snippet: A) Vertical sections from the central retina of C57BL/6J mice were subjected to immunostaining with GlyT1 and GAD67 antibodies followed by incubation with CY-3 and Alexa-488 labeled secondary antibodies, respectively. Scale bar, 50 μm. A1 and A2) Magnification of the areas enclosed in panel A, cells labeled for GAD67 and GlyT1 are marked by an arrow. B) Vertical sections were stained with HPC-1, GlyT1 and GAD67 and the resulting images used for quantitation as described in ​in4D.4D. C) Vertical sections from the central retina of the transgenic line pGAD67-EGFP were labeled with GlyT1 antibody from Synaptic Systems followed by incubation with CY-3 labeled secondary antibodies. Nuclei were stained with DAPI. Arrows indicate co-expression of GlyT1 and eGFP in amacrine neurons. Scale bar, 10 μm for B and C. D) Sections from the pGAD67-EGFP were subjected to quantitation analysis, as described in ​in4D.4D. Scale bar, 10 μm.

Article Snippet: Anti-Mouse (CY3) , Donkey , Jackson Immunoresearch Laboratories, 715–165-150 , .

Techniques: Immunostaining, Incubation, Labeling, Staining, Quantitation Assay, Transgenic Assay, Expressing

Journal: Medical research archives

Article Title: Identification of amacrine neurons with a glycinergic and GABAergic phenotype in the mouse retina

doi: 10.18103/mra.v10i1.2624

Figure Lengend Snippet: A) Primary dissociated retinal cultures were fixed with paraformaldehyde and incubated with antibodies recognizing GAD67 and GlyT1 followed by staining with the corresponding CY-3 and CY-5 labeled antibodies, respectively. The inhibitory neurons were heterogeneous and contained eGFP and GlyT1 (long arrows), or eGFP, GAD67 and GlyT1 (open arrowheads), or GAD67 (thin open arrows). B) Cells were subjected to immunostaining with antibodies against GAT1 and GlyT1 or C) against GAT3 and GlyT1 as described under Experimental Procedures. Scale bars, 10 μm.

Article Snippet: Anti-Mouse (CY3) , Donkey , Jackson Immunoresearch Laboratories, 715–165-150 , .

Techniques: Incubation, Staining, Labeling, Immunostaining